Found 80 of ic50 for monomerid = 50396483 TargetSerine/threonine-protein kinase A-Raf(Homo sapiens (Human))
China Pharmaceutical University
Curated by ChEMBL
China Pharmaceutical University
Curated by ChEMBL
Affinity DataIC50: 2.10nMAssay Description:Inhibition of ARaf (unknown origin)More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase A-Raf(Homo sapiens (Human))
China Pharmaceutical University
Curated by ChEMBL
China Pharmaceutical University
Curated by ChEMBL
Affinity DataIC50: 2.10nMAssay Description:Inhibition of ARAF (unknown origin) using human His6-tagged MEK1 K97R mutant as substrate pretreated for 20 mins followed by [33P]-ATP addition measu...More data for this Ligand-Target Pair
Affinity DataIC50: 4nMAssay Description:Inhibition of B-Raf V600E mutant (unknown origin) assessed as MEK1 phosphorylation using MEK1-Avitag as substrate after 1 hr by HTRF assayMore data for this Ligand-Target Pair
3D Structure (crystal)Affinity DataIC50: 6.10nMAssay Description:Inhibition of recombinant BRAF V600E mutant (unknown origin) assessed as ADP formation measured for 5 hrs by pyruvate kinase/lactate dehydrogenase co...More data for this Ligand-Target Pair
Affinity DataIC50: 6.90nMAssay Description:Inhibition of BRAF (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 6.90nMAssay Description:Inhibition of wild-type BRAF (unknown origin) using human His6-tagged MEK1 K97R mutant as substrate pretreated for 20 mins followed by [33P]-ATP addi...More data for this Ligand-Target Pair
Affinity DataIC50: 9.60nMAssay Description:Inhibition of human recombinant BRAF V600E mutant using MEK1 as substrate measured after 1 hr by Western blot analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 17nMAssay Description:Inhibition of B-Raf V600E mutant in human A375 cells assessed as ERK phosphorylation preincubated for 1 hr by Western blot methodMore data for this Ligand-Target Pair
Affinity DataIC50: 20nMAssay Description:Inhibition of wild type B-Raf (unknown origin) assessed as MEK1 phosphorylation using MEK1-Avitag as substrate after 1 hr by HTRF assayMore data for this Ligand-Target Pair
Affinity DataIC50: 21nMAssay Description:Inhibition of wild type BRAF (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 21nMAssay Description:Inhibition of BRAF V600E mutant (unknown origin) after 20 mins by immunoblotting assayMore data for this Ligand-Target Pair
Affinity DataIC50: 21nMAssay Description:Inhibition of recombinant His-tagged BRAF V600E mutant (unknown origin) (444-723 residues) expressed in Escherichia coli BL21(DE3)-RIL using MEKK97R ...More data for this Ligand-Target Pair
Affinity DataIC50: 23nMAssay Description:Inhibition of human GST-tagged full length B-Raf V600E mutant (unknown origin) using His6-tagged full-length human MEK1 (K97R) substrate and [33P]-ga...More data for this Ligand-Target Pair
TargetMitogen-activated protein kinase kinase kinase 20(Homo sapiens (Human))
University Of Macau
Curated by ChEMBL
University Of Macau
Curated by ChEMBL
Affinity DataIC50: 23nMAssay Description:Inhibition of human ZAK (5 to 309 residues) expressed in baculovirus infected Sf9 insect cells using ZAKtide as substrate after 1 hr by mass spectrom...More data for this Ligand-Target Pair
Affinity DataIC50: 30nMAssay Description:Inhibition of mouse full length GST-tagged BRAF V600E mutant using recombinant human full length N-terminal His-tagged MEK1 as substrate preincubated...More data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of N-terminal his-tagged BRAF V600E mutant (448 to 723 residues) (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed a...More data for this Ligand-Target Pair
TargetMitogen-activated protein kinase kinase kinase 20(Homo sapiens (Human))
University Of Macau
Curated by ChEMBL
University Of Macau
Curated by ChEMBL
Affinity DataIC50: 31nMAssay Description:Inhibition of N-terminal GST-tagged recombinant human full-length ZAK expressed in baculovirus infected Sf9 insect cells using MBP as substrate after...More data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of N-terminal His-tagged BRAF V600E mutant (unknown origin) expressed in baculovirus infected insect cells co-expressing CDC37 using bioti...More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf [V600E](Homo sapiens (Human))
Korea Institute Of Science And Technology
US Patent
Korea Institute Of Science And Technology
US Patent
Affinity DataIC50: 31nMAssay Description:Reaction biology kinase hotspot service (http://www.reactionbiology.com) was used to measure IC50. In a final reaction volume of 25 μL, kinase (...More data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of full length human B-Raf V600E mutant expressed in baculovirus infected insect cells assessed as [gamma-33P]incorporation into MEK after...More data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of B-Raf (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of recombinant human GST-tagged BRAF V600E mutant expressed in baculovirus expression systemMore data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of BRAF (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of BRAF V600E mutant (unknown origin)More data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf [V600E](Homo sapiens (Human))
Korea Institute Of Science And Technology
US Patent
Korea Institute Of Science And Technology
US Patent
Affinity DataIC50: 31nMAssay Description:Reaction biology kinase hotspot service (http://www.reactionbiology.com) was used to measure IC50. In a final reaction volume of 25 μL, kinase (...More data for this Ligand-Target Pair
Affinity DataIC50: 31nMAssay Description:Inhibition of human BRAF V600E mutant using MEK1 as substrate in presence of ATPMore data for this Ligand-Target Pair
Affinity DataIC50: 32nMAssay Description:Inhibition of human GST-tagged BRAF V600E mutant (416 to 766 residues) using human full length 6His-tagged MEK1 (K97R) as substrate preincubated for ...More data for this Ligand-Target Pair
Affinity DataIC50: 32nMAssay Description:Inhibition of BRAF V600E mutant in human A375 cells assessed as reduction in ERK phosphorylation by AlphaScreen assayMore data for this Ligand-Target Pair
Affinity DataIC50: 33nMAssay Description:Inhibition of B-raf V600E mutant in human A375 cells assessed as reduction in ERK1/2 phosphorylation incubated for 90 mins by Western blotting methodMore data for this Ligand-Target Pair
Affinity DataIC50: 33nMAssay Description:Inhibition of human recombinant B-Raf V600E mutant by FRET-based Z'-lyte assayMore data for this Ligand-Target Pair
Affinity DataIC50: 34nMAssay Description:Inhibition of wild type BRAF (unknown origin) assessed as ADP formation measured for 5 hrs by pyruvate kinase/lactate dehydrogenase coupled assay in ...More data for this Ligand-Target Pair
TargetEpidermal growth factor receptor [696-1022,L858R](Homo sapiens (Human))
Korea Institute Of Science And Technology
US Patent
Korea Institute Of Science And Technology
US Patent
Affinity DataIC50: 48nMAssay Description:Reaction biology kinase hotspot service (http://www.reactionbiology.com) was used to measure IC50. In a final reaction volume of 25 μL, kinase (...More data for this Ligand-Target Pair
TargetRAF proto-oncogene serine/threonine-protein kinase(Homo sapiens (Human))
Korea Institute Of Science And Technology
US Patent
Korea Institute Of Science And Technology
US Patent
Affinity DataIC50: 48nMAssay Description:Reaction biology kinase hotspot service (http://www.reactionbiology.com) was used to measure IC50. In a final reaction volume of 25 μL, kinase (...More data for this Ligand-Target Pair
TargetRAF proto-oncogene serine/threonine-protein kinase(Homo sapiens (Human))
Korea Institute Of Science And Technology
US Patent
Korea Institute Of Science And Technology
US Patent
Affinity DataIC50: 48nMAssay Description:Inhibition of C-Raf in presence of 100 uM ATPMore data for this Ligand-Target Pair
Affinity DataIC50: 59nMAssay Description:Inhibition of recombinant human N-terminal GST-tagged B-Raf V600E mutant expressed in baculovirus expression system using Z'-LYTE Ser/Thr3 peptide as...More data for this Ligand-Target Pair
Affinity DataIC50: 61nMAssay Description:Inhibition of B-Raf V600E mutant-mediated Erk phosphorylation in human MALME-3M cells after 1 hr by fluorescence analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 80nMAssay Description:Inhibition of BRAF V600E in human A375 cells assessed as reduction in ERK phosphorylation incubated for 1 hr by immunoblot analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 84nMAssay Description:Inhibition of wild type GST-tagged recombinant human full-length B-RAF expressed in baculovirus expression system using Ser/Thr3 as substrate after 1...More data for this Ligand-Target Pair
Affinity DataIC50: 100nMAssay Description:Inhibition of recombinant human full length GST-tagged BRAF expressed in baculovirus expression systemMore data for this Ligand-Target Pair
Affinity DataIC50: 100nMAssay Description:Reaction biology kinase hotspot service (http://www.reactionbiology.com) was used to measure IC50. In a final reaction volume of 25 μL, kinase (...More data for this Ligand-Target Pair
Affinity DataIC50: 100nMAssay Description:Reaction biology kinase hotspot service (http://www.reactionbiology.com) was used to measure IC50. In a final reaction volume of 25 μL, kinase (...More data for this Ligand-Target Pair
Affinity DataIC50: 100nMAssay Description:Inhibition of B-Raf in presence of 100 uM ATPMore data for this Ligand-Target Pair
TargetSerine/threonine-protein kinase B-raf [V600E](Homo sapiens (Human))
Korea Institute Of Science And Technology
US Patent
Korea Institute Of Science And Technology
US Patent
Affinity DataIC50: 107nMAssay Description:ormula (I) bis-hydrochloride salt prepared in Example 2 (hereinafter referred to as “Compound⋅2HCl”) was tested for inhibitory activity against...More data for this Ligand-Target Pair
Affinity DataIC50: 111nMAssay Description:ormula (I) bis-hydrochloride salt prepared in Example 2 (hereinafter referred to as “Compound⋅2HCl”) was tested for inhibitory activity against...More data for this Ligand-Target Pair
Affinity DataIC50: 119nMAssay Description:Inhibition of recombinant wild type full length human GST-tagged B-Raf expressed in baculovirus expression system using Z'-LYTE Ser/Thr3 peptide as s...More data for this Ligand-Target Pair
Affinity DataIC50: 127nMAssay Description:Inhibition of BRAF V600E mutant in human A375 cells assessed as reduction in cell proliferation incubated for 96 hrs by MTT assayMore data for this Ligand-Target Pair
TargetRAF proto-oncogene serine/threonine-protein kinase [Y340D,Y341D](Homo sapiens (Human))
Hanmi Pharm.
US Patent
Hanmi Pharm.
US Patent
Affinity DataIC50: 128nMAssay Description:The compounds prepared in Examples were tested for inhibitory activities against three subtypes of RAF, i.e. RAF1 Y340D Y341D, BRAF normal type and B...More data for this Ligand-Target Pair
TargetRAF proto-oncogene serine/threonine-protein kinase [Y340D,Y341D](Homo sapiens (Human))
Hanmi Pharm.
US Patent
Hanmi Pharm.
US Patent
Affinity DataIC50: 128nMAssay Description:The compounds prepared in Examples were tested for inhibitory activity against three subtypes of RAF, i.e., RAF1 Y340D Y341D (C-RAF), B-RAF normal ty...More data for this Ligand-Target Pair
TargetRAF proto-oncogene serine/threonine-protein kinase(Homo sapiens (Human))
Korea Institute Of Science And Technology
US Patent
Korea Institute Of Science And Technology
US Patent
Affinity DataIC50: 135nMAssay Description:Inhibition of cRAF (unknown origin)More data for this Ligand-Target Pair
TargetRAF proto-oncogene serine/threonine-protein kinase(Homo sapiens (Human))
Korea Institute Of Science And Technology
US Patent
Korea Institute Of Science And Technology
US Patent
Affinity DataIC50: 135nMAssay Description:Inhibition of CRAF (unknown origin) using human His6-tagged MEK1 K97R mutant as substrate pretreated for 20 mins followed by [33P]-ATP addition measu...More data for this Ligand-Target Pair
