Affinity DataKi: 16.2nMAssay Description:Displacement of [3H]cyclopamine from wild type Smo expressed in U2OS cells after 2 hrs by scintillation countingMore data for this Ligand-Target Pair
Affinity DataIC50: 1.5nMAssay Description:Antagonist activity at Smo in mouse Shh-Light 2 cells assessed as inhibition of Shh-induced Gli1-reporter activity after 2 days by dual-luciferase re...More data for this Ligand-Target Pair
Affinity DataIC50: 5nMAssay Description:Inhibition of Smo in mouse C3H10T1/2 cells using human recombinant SHH assessed as effect on SMO/SHH transient transcriptional activation after 20 hr...More data for this Ligand-Target Pair
Affinity DataIC50: 6nMAssay Description:Inhibition of smo-mediated hedgehog signaling pathway in mouse NIH3T3 cells expressing GRE-Luc reporter gene assessed as inhibition of SAG-induced GR...More data for this Ligand-Target Pair
Affinity DataIC50: 7nMAssay Description:Displacement of BODIPY-labelled cyclopamine from human Smo receptor expressed in HEK293 cells after 2 hrs by fluorescence microscopyMore data for this Ligand-Target Pair
Affinity DataIC50: 7nMAssay Description:Inhibition of Smo-mediated Hh signaling in human Shh-light2 cells by luciferase reporter gene assayMore data for this Ligand-Target Pair
Affinity DataIC50: 16nMAssay Description:Inhibition of SMO in mouse NIH/3T3 cells assessed as inhibition of SAG-induced hedgehog-mediated luminescence signaling by GRE-luciferase reporter ge...More data for this Ligand-Target Pair
Affinity DataIC50: 20nMAssay Description:Inhibition of smoothened (unknown origin)-mediated Shh signalingMore data for this Ligand-Target Pair
Affinity DataIC50: 23nMAssay Description:Inhibition of Smo receptor (unknown origin) expressed in NIH3T3 cells assessed as inhibition of Smo agonist SAG-induced GRE activation after 30 hrs b...More data for this Ligand-Target Pair
Affinity DataIC50: 33nMAssay Description:Antagonist activity at smoothened (unknown origin) expressed in mouse Shh Light2 cells co-expressing Gli-dependent reporter gene assessed as inhibiti...More data for this Ligand-Target Pair
Affinity DataIC50: 39nMAssay Description:Inhibition of Smo-mediated Hh signalling pathway in mouse Shh Light2 cells by Gli-luciferase reporter gene assayMore data for this Ligand-Target Pair
Affinity DataIC50: 46nMAssay Description:Antagonist activity at Smo receptor in mouse NIH/3T3 cells harboring GRE-Luc assessed as inhibition of SAG-induced Hh signaling pathway preincubated ...More data for this Ligand-Target Pair