Reaction Details  Report a problem with these data
Cell Reactant:
Transcriptional Regulator TtgR Mutant R176G
Syringe Reactant:
BDBM19461
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
07/23/08
ΔG°:
-26.334±2.508 (kJ/mole)
pH:
7±n/a
Log10Kb:
3.5
Temperature:
303.15±n/a (K)
ΔHobs :
-104.082±14.63 (kJ/mole)
Corrected for ΔHioniz:
no
ΔS° :
-0.2565±0.0248 (kJ/mole-K)
Citation
 Alguel, Y; De Lucca, GV; Liang, J; Meng, C; Terán, W; De Lucca, I; Krell, T; Ramos, JL; Gallegos, MT; Zhang, X Crystal structures of multidrug binding protein TtgR in complex with antibiotics and plant antimicrobials. J Mol Biol 369:829-40 (2007) [PubMed]  Article
Cell React
Source:
Site-directed mutant was cloned, and protein was expressed and purified from E. coli.
Prep. Method:
TtgR mutant protein was further purified by size exclusion chromatography using a Sephacryl HR-200 column (Amersham Biosciences). Eluted fractions of TtgR were pooled, concentrated, and dialyzed against the buffer.
Name:
Transcriptional Regulator TtgR Mutant R176G
Synonyms:
HTH-type transcriptional regulator ttgR | Toluene efflux pump ttgABC operon repressor
Type:
Repressor; homodimer
Mol. Mass.:
23752.43
Organism:
Pseudomonas putida
Description:
n/a
Residue:
210
Sequence:
MVRRTKEEAQETRAQIIEAAERAFYKRGVARTTLADIAELAGVTRGAIYWHFNNKAELVQALLDSLHETHDHLARASESEDEVDPLGCMRKLLLQVFNELVLDARTRRINEILHHKCEFTDDMCEIRQQRQSAVLDCHKGITLALANAVRRGQLPGELDAERAAVAMFAYVDGLIGRWLLLPDSVDLLGDVEKWVDTGLDMLRLSPALRK
  
Syringe React
Name:
BDBM19461
Synonyms:
α-CA inhibitor, 5 | 5,7-dihydroxy-2-(4-hydroxyphenyl)-3,4-dihydro-2H-1-benzopyran-4-one | CHEMBL32571 | Naringenin | Naringenin (NAR) | naringetol | salipurpol
Type:
Estrgen Antagonist
Emp. Form.:
C15H12O5
Mol. Mass.:
272.2528
SMILES:
Oc1ccc(cc1)C1CC(=O)c2c(O)cc(O)cc2O1
Structure:
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