| Reaction Details |
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| Report a problem with these data |
| Target | Protein kinase B (Akt 2) |
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| Ligand | A-443654 |
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| Substrate/Competitor | AKTide-2T |
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| Meas. Tech. | PKB In Vitro Enzyme Assay |
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| pH | 7.2±n/a |
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| Temperature | 295.15±n/a K |
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| IC50 | 0.5±n/a nM |
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| Citation |  Davies, TG; Verdonk, ML; Graham, B; Saalau-Bethell, S; Hamlett, CC; McHardy, T; Collins, I; Garrett, MD; Workman, P; Woodhead, SJ; Jhoti, H; Barford, D A structural comparison of inhibitor binding to PKB, PKA and PKA-PKB chimera.J Mol Biol.367:882-94(2007)[PubMed] |
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| More Info. |
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, Solution Info
, Assay Method
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| Protein kinase B (Akt 2) |
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| Name | Protein kinase B (Akt 2) |
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| Synonyms | EC 2.7.11.1 | RAC-PK-beta | Protein kinase Akt-2 | Protein kinase B, beta | PKB beta | RAC-beta serine/threonine-protein kinase |
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| Type | Enzyme |
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| Mol. Mass. | n/a |
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| Organism | Homo sapiens (human) |
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| Description | PKBbeta-PIFtide construct expressed in Sf9 cell. |
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| Residue | 342 |
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| Sequence | GAMDPKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTE
SRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGELFFHLSRERVFTEERARFYGAEIV
SALEYLHSRDVVYRDIKLENLMLDKDGHIKITDFGLCKEGISDGATMKTFCGTPEYLAPE
VLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHERLFELILMEEIRFPRTLSPEAKSL
LAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQKKLLPPFKPQVTSEVDTRYFD
DEFTAQSITITPPDRYDSLGLLELDQREEQEMFEDFDYIADW
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| A-443654 |
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| Name | A-443654 |
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| Synonyms: | 5-indazolyl pyridine 3 | 5-{5-[(2S)-2-amino-3-(1H-indol-3-yl)propoxy]pyridin-3-yl}-3-methyl-1H-indazole | indazole-pyridine, 4 |
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| Type | Small organic molecule |
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| Emp. Form. | C24H23N5O |
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| Mol. Mass. | 397.4723 |
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| SMILES | Cc1n[nH]c2ccc(cc12)-c1cncc(OC[C@@H](N)Cc2c[nH]c3ccccc23)c1 |r| |
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| Structure | |
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| AKTide-2T |
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| Name | AKTide-2T |
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| Synonyms | n/a |
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| Type | Peptide |
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| Mol. Mass. | n/a |
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| Organism | n/a |
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| Description | n/a |
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| Residue | 14 |
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| Sequence | |
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