| Reaction Details |
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| Target | Purine Nucleoside Phosphorylase (PNP) |
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| Ligand | Azetidine based compound, (+/-) 42 |
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| Substrate/Competitor | Inosine |
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| Meas. Tech. | PNP Inhibition Assay |
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| pH | 7.7±n/a |
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| Temperature | 295.15±n/a K |
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| Ki | 1.8±0.2 nM |
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| Km | 34000±n/a nM |
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| Citation |  Evans, GB; Furneaux, RH; Greatrex, B; Murkin, AS; Schramm, VL; Tyler, PC Azetidine based transition state analogue inhibitors of N-ribosyl hydrolases and phosphorylases.J Med Chem.51:948-56(2008)[PubMed] |
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| More Info. |
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, Solution Info
, Assay Method
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| Purine Nucleoside Phosphorylase (PNP) |
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| Name | Purine Nucleoside Phosphorylase (PNP) |
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| Synonyms | EC 2.4.2.1 | Inosine phosphorylase |
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| Type | Enzyme |
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| Mol. Mass. | 32037 |
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| Organism | Bos taurus (bovine) |
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| Description | n/a |
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| Residue | 289 |
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| Sequence | MANGYTYEDYQDTAKWLLSHTEQRPQVAVICGSGLGGLVNKLTQAQTFDYSEIPNFPEST
VPGHAGRLVFGILNGRACVMMQGRFHMYEGYPFWKVTFPVRVFRLLGVETLVVTNAAGGL
NPNFEVGDIMLIRDHINLPGFSGENPLRGPNEERFGVRFPAMSDAYDRDMRQKAHSTWKQ
MGEQRELQEGTYVMLGGPNFETVAECRLLRNLGADAVGMSTVPEVIVARHCGLRVFGFSL
ITNKVIMDYESQGKANHEEVLEAGKQAAQKLEQFVSLLMASIPVSGHTG
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| Azetidine based compound, (+/-) 42 |
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| Name | Azetidine based compound, (+/-) 42 |
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| Synonyms: | 7-{[2-(hydroxymethyl)azetidin-1-yl]methyl}-3H,4H,5H-pyrrolo[3,2-d]pyrimidin-4-one |
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| Type | Small organic molecule |
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| Emp. Form. | C11H14N4O2 |
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| Mol. Mass. | 234.2545 |
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| SMILES | OCC1CCN1Cc1c[nH]c2c1nc[nH]c2=O |
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| Structure | |
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| Inosine |
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| Name | Inosine |
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| Synonyms: | 9-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-6,9-dihydro-3H-purin-6-one | Atorel | Hypoxanthosine |
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| Type | Purine Nucleoside |
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| Emp. Form. | C10H12N4O5 |
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| Mol. Mass. | 268.2261 |
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| SMILES | OC[C@H]1O[C@H]([C@H](O)[C@@H]1O)n1cnc2c1[nH]cnc2=O |
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| Structure | |
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