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TargetRunt-Related Transcription Factor 1 Isoform AML1C
LigandBDBM29665
Substrate/CompetitorVenus-CBFbeta-SMMHC
Meas. Tech.uHTS identification of compounds inhibiting the binding between the RUNX1 Runt domain and CBFb-SMMHC via a fluorescence resonance energy transfer (FRET) assay.
pH7.4±n/a
Temperature296.15±n/a K
IC50 13000±n/a nM
Citation PubChem, PC uHTS identification of compounds inhibiting the binding between the RUNX1 Runt domain and CBFbeta-SMMHC via a fluorescence resonance energy transfer (FRET) assay. PubChem Bioassay(2009)[AID]
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Runt-Related Transcription Factor 1 Isoform AML1C
Name:Runt-Related Transcription Factor 1 Isoform AML1C
Synonyms:Acute myeloid leukemia 1 protein | CBF-alpha-2 | Core-binding factor subunit alpha-2 | Oncogene AML-1 | Polyomavirus enhancer-binding protein 2 alpha B subunit
Type:Subunit of heterodimeric transcription factor
Mol. Mass.:48750.25
Organism:Homo sapiens (Human)
Description:Q01196
Residue:453
Sequence:
MRIPVDASTSRRFTPPSTALSPGKMSEALPLGAPDAGAALAGKLRSGDRSMVEVLADHPG
ELVRTDSPNFLCSVLPTHWRCNKTLPIAFKVVALGDVPDGTLVTVMAGNDENYSAELRNA
TAAMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKITVDGPREPRRHR
QKLDDQTKPGSLSFSERLSELEQLRRTAMRVSPHHPAPTPNPRASLNHSTAFNPQPQSQM
QDTRQIQPSPPWSYDQSYQYLGSIASPSVHPATPISPGRASGMTTLSAELSSRLSTAPDL
TAFSDPRQFPALPSISDPRMHYPGAFTYSPTPVTSGIGIGMSAMGSATRYHTYLPPPYPG
SSQAQGGPFQASSPSYHLYYGASAGSYQFSMVGGERSPPRILPPCTNASTGSALLNPSLP
NQSDVVEAEGSHSNSPTNMAPSARLEEAVWRPY
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BDBM29665
NameBDBM29665
Synonyms:(9-Oxo-9H-acridin-10-yl)-acetic acid (2,5-dimethyl-1-p-tolyl-1H-pyrrol-3-ylmethylene)-hydrazide | MLS000566526 | N-[[2,5-dimethyl-1-(4-methylphenyl)-3-pyrrolyl]methylideneamino]-2-(9-oxo-10-acridinyl)acetamide | N-[[2,5-dimethyl-1-(4-methylphenyl)pyrrol-3-yl]methylideneamino]-2-(9-oxidanylideneacridin-10-yl)ethanamide | N-[[2,5-dimethyl-1-(4-methylphenyl)pyrrol-3-yl]methylideneamino]-2-(9-oxoacridin-10-yl)acetamide | N-[[2,5-dimethyl-1-(p-tolyl)pyrrol-3-yl]methyleneamino]-2-(9-ketoacridin-10-yl)acetamide | SMR000175922 | cid_3116773
TypeSmall organic molecule
Emp. Form.C29H26N4O2
Mol. Mass.462.5423
SMILESCc1cc(C=NNC(=O)Cn2c3ccccc3c(=O)c3ccccc23)c(C)n1-c1ccc(C)cc1 |w:5.5|
Structure
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Venus-CBFbeta-SMMHC
Name:Venus-CBFbeta-SMMHC
Synonyms:CBFbeta | Core-binding factor subunit beta isoform 2 | Venus-core-binding factor subunit beta-smooth muscle myosin heavy chain
Type:Fluorescent fusion protein
Mol. Mass.:21988.19
Organism:Homo sapiens (Human)
Description:Venus-CBFbeta-SMMHC was provided by Dr. John Bushweller (University of Virginia Charlottesville, Charlottesville VA). Only the amino acid sequence of core-binding factor subunit beta (CBFbeta) isoform 2 is shown here.
Residue:187
Sequence:
MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVAT
GTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLD
GMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEARRQQDPSPGSNLGG
GDDLKLR
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