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TargetRunt-Related Transcription Factor 1 Isoform AML1C
LigandBDBM29682
Substrate/CompetitorVenus-CBFbeta-SMMHC
Meas. Tech.uHTS identification of compounds inhibiting the binding between the RUNX1 Runt domain and CBFb-SMMHC via a fluorescence resonance energy transfer (FRET) assay.
pH7.4±n/a
Temperature296.15±n/a K
IC50 13400±n/a nM
Citation PubChem, PC uHTS identification of compounds inhibiting the binding between the RUNX1 Runt domain and CBFbeta-SMMHC via a fluorescence resonance energy transfer (FRET) assay. PubChem Bioassay(2009)[AID]
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Runt-Related Transcription Factor 1 Isoform AML1C
Name:Runt-Related Transcription Factor 1 Isoform AML1C
Synonyms:Acute myeloid leukemia 1 protein | CBF-alpha-2 | Core-binding factor subunit alpha-2 | Oncogene AML-1 | Polyomavirus enhancer-binding protein 2 alpha B subunit
Type:Subunit of heterodimeric transcription factor
Mol. Mass.:48750.25
Organism:Homo sapiens
Description:Q01196
Residue:453
Sequence:
MRIPVDASTSRRFTPPSTALSPGKMSEALPLGAPDAGAALAGKLRSGDRSMVEVLADHPG
ELVRTDSPNFLCSVLPTHWRCNKTLPIAFKVVALGDVPDGTLVTVMAGNDENYSAELRNA
TAAMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKITVDGPREPRRHR
QKLDDQTKPGSLSFSERLSELEQLRRTAMRVSPHHPAPTPNPRASLNHSTAFNPQPQSQM
QDTRQIQPSPPWSYDQSYQYLGSIASPSVHPATPISPGRASGMTTLSAELSSRLSTAPDL
TAFSDPRQFPALPSISDPRMHYPGAFTYSPTPVTSGIGIGMSAMGSATRYHTYLPPPYPG
SSQAQGGPFQASSPSYHLYYGASAGSYQFSMVGGERSPPRILPPCTNASTGSALLNPSLP
NQSDVVEAEGSHSNSPTNMAPSARLEEAVWRPY
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BDBM29682
NameBDBM29682
Synonyms:2-(dichloromethyl)-7-(phenylmethyl)-3,5,6,8-tetrahydropyrido[2,3]thieno[2,4-b]pyrimidin-4-one | 2-[bis(chloranyl)methyl]-7-(phenylmethyl)-3,5,6,8-tetrahydropyrido[2,3]thieno[2,4-b]pyrimidin-4-one | 7-benzyl-2-(dichloromethyl)-3,5,6,8-tetrahydropyrido[2,3]thieno[2,4-b]pyrimidin-4-one | 7-benzyl-2-(dichloromethyl)-5,6,7,8-tetrahydropyrido[4',3':4,5]thieno[2,3-d]pyrimidin-4(3H)-one | MLS000705398 | SMR000232051 | cid_986648
TypeSmall organic molecule
Emp. Form.C17H15Cl2N3OS
Mol. Mass.380.292
SMILESClC(Cl)c1nc2sc3CN(Cc4ccccc4)CCc3c2c(=O)[nH]1
Structure
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Venus-CBFbeta-SMMHC
Name:Venus-CBFbeta-SMMHC
Synonyms:CBFbeta | Core-binding factor subunit beta isoform 2 | Venus-core-binding factor subunit beta-smooth muscle myosin heavy chain
Type:Fluorescent fusion protein
Mol. Mass.:21988.19
Organism:Homo sapiens (human)
Description:Venus-CBFbeta-SMMHC was provided by Dr. John Bushweller (University of Virginia Charlottesville, Charlottesville VA). Only the amino acid sequence of core-binding factor subunit beta (CBFbeta) isoform 2 is shown here.
Residue:187
Sequence:
MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVAT
GTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLD
GMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEARRQQDPSPGSNLGG
GDDLKLR
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