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TargetReplicase polyprotein 1ab [1541-1855]
LigandBDBM31524
Substrate/CompetitorSARS-CoV PLpro Substrate Peptide
Meas. Tech.SARS-CoV PLpro Enzyme Inhibition Assay
pH7.5±n/a
Temperature296.15±n/a K
IC50 2640±40 nM
Citation Ghosh, AKTakayama, JAubin, YRatia, KChaudhuri, RBaez, YSleeman, KCoughlin, MNichols, DBMulhearn, DCPrabhakar, BSBaker, SCJohnson, MEMesecar, AD Structure-Based Design, Synthesis, and Biological Evaluation of a Series of Novel and Reversible Inhibitors for the Severe Acute Respiratory Syndrome-Coronavirus Papain-Like Protease. J Med Chem52:5228-40 (2009) [PubMed]  Article
More Info.:Get all data from this article,  Inhibition_Run data,   Solution Info,  Assay Method
 
Replicase polyprotein 1ab [1541-1855]
Name:Replicase polyprotein 1ab [1541-1855]
Synonyms:SARS Coronavirus Coronavirus Papain-Like Protease | SARS-CoV PLpro | SARS-CoV papain-like protease (SARS-PLpro)
Type:Hydrolase
Mol. Mass.:35352.30
Organism:Human SARS coronavirus (SARS-CoV)
Description:The SARS-CoV PLpro enzyme (residues 1541 -1855) was expressed and purified from E. coli.
Residue:315
Sequence:
EVKTIKVFTTVDNTNLHTQLVDMSMTYGQQFGPTYLDGADVTKIKPHVNHEGKTFFVLPS
DDTLRSEAFEYYHTLDESFLGRYMSALNHTKKWKFPQVGGLTSIKWADNNCYLSSVLLAL
QQLEVKFNAPALQEAYYRARAGDAANFCALILAYSNKTVGELGDVRETMTHLLQHANLES
AKRVLNVVCKHCGQKTTTLTGVEAVMYMGTLSYDNLKTGVSIPCVCGRDATQYLVQQESS
FVMMSAPPAEYKLQQGTFLCANEYTGNYQCGHYTHITAKETLYRIDGAHLTKMSEYKGPV
TDVFYKETSYTTTIK
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM31524
NameBDBM31524
Synonyms:5-amino-2-methyl-N-[(1R)-1-(naphthalen-1-yl)ethyl]benzamide (I-1) | AKOS015840255 | GRL0617 | Naphthalene and Benzamide Derivative, 25 | SARS-CoV-2 PLpro Inhibitor, GRL0617 (1) | med.21724, Compound 159
TypeSmall organic molecule
Emp. Form.C20H20N2O
Mol. Mass.304.3856
SMILESC[C@@H](NC(=O)c1cc(N)ccc1C)c1cccc2ccccc12 |r|
Structure
SARS-CoV PLpro Substrate Peptide
Name:SARS-CoV PLpro Substrate Peptide
Synonyms:Arg-Leu-Arg-Gly-Gly-AMC
Type:Fluorogenic peptide
Mol. Mass.:865.08
Organism:n/a
Description:n/a
Residue:8
Sequence:
RLRGG-AMC