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TargetSrc Mutant (S345C)
LigandBDBM4583
Substrate/CompetitorTyr 02 Substrate Peptide
Meas. Tech.Src IC50 Determination
pH7.5±n/a
Temperature296.15±n/a K
IC50 236±n/a nM
Citation Michalczyk, AKlüter, SRode, HBSimard, JRGrütter, CRabiller, MRauh, D Structural insights into how irreversible inhibitors can overcome drug resistance in EGFR. Bioorg Med Chem16:3482-8 (2008) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Src Mutant (S345C)
Name:Src Mutant (S345C)
Synonyms:Proto-oncogene tyrosine-protein kinase Src | cSRC-SM
Type:Tyr protein kinase
Mol. Mass.:32702.38
Organism:Gallus gallus (Chicken)
Description:Mutation of S345 to cysteine was introduced by site-directed mutagenesis and confirmed by sequence analysis.
Residue:286
Sequence:
GHMQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAF
LQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGCLLDFLKGEMGKYLRLPQLVDMA
AQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIK
WTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPEC
PESLHDLMCQCWRKDPEERPTFEYLQAFLEDYFTSTEPQYQPGENL
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM4583
NameBDBM4583
Synonyms:(2E)-N-{4-[(3-bromophenyl)amino]quinazolin-6-yl}-4-(dimethylamino)but-2-enamide | 4-anilinoquinazoline deriv. 16a | BMC163482 Compound 2 | CHEMBL94061
TypeSmall organic molecule
Emp. Form.C20H20BrN5O
Mol. Mass.426.31
SMILESCN(C)C\C=C\C(=O)Nc1ccc2ncnc(Nc3cccc(Br)c3)c2c1
Structure
Search PDB for entries with ligand similarity:Similarity to this molecule at least:
Tyr 02 Substrate Peptide
Name:Tyr 02 Substrate Peptide
Synonyms:n/a
Type:FRET-peptide substrate
Mol. Mass.:358.43
Organism:n/a
Description:n/a
Residue:3
Sequence:
n/a