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TargetGlucocerebrosidase (Acid beta-Glucosidase)
LigandBDBM36514
Substrate/CompetitorBDBM18352
Meas. Tech.Enzymatic Activity Assay
pH5.2±0
Temperature310.15±0 K
Ki 7± 2 nM
IC50 1.24±0.04 nM
Citation Witte, MDKallemeijn, WWAten, JLi, KYStrijland, ADonker-Koopman, WEvan den Nieuwendijk, AMBleijlevens, BKramer, GFlorea, BIHooibrink, BHollak, CEOttenhoff, RBoot, RGvan der Marel, GAOverkleeft, HSAerts, JM Ultrasensitive in situ visualization of active glucocerebrosidase molecules. Nat Chem Biol6:907-13 (2010) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
Glucocerebrosidase (Acid beta-Glucosidase)
Name:Glucocerebrosidase (Acid beta-Glucosidase)
Synonyms:β-glucocerebrosidase (GCase) | Acid beta-glucosidase | Alglucerase | Beta-glucocerebrosidase (GC) | D-glucosyl-N-acylsphingosine glucohydrolase | Glucocerebrosidase (GBA) | Glucosylceramidase (GBA) | Glucosylceramidase (GCase) | Glucosylceramidase precursor (Beta-glucocerebrosidase) (Acid beta-glucosidase) (D-glucosyl-N-acylsphingosine glucohydrolase) (Alglucerase) (Imiglucerase). | Imiglucerase
Type:Enzyme
Mol. Mass.:59724.64
Organism:Homo sapiens (human)
Description:The beta-Glu activity was measured with commercially available beta-glucocerebrosidase (Ceredase) as the enzyme source.
Residue:536
Sequence:
MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNAT
YCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGF
GGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDD
FQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQP
GDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIA
RDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAK
ATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDW
NLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQK
NDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ
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BDBM36514
BDBM18352
NameBDBM36514
Synonyms:CID46912122 | MDW933, 5
TypeSmall organic molecule
Emp. Form.C26H34BF2N5O4
Mol. Mass.529.387
SMILESCC1=CC(C)=[N+]2C1=C(CCCCc1cn(C[C@H]3[C@H]4O[C@H]4[C@H](O)[C@@H](O)[C@@H]3O)nn1)c1c(C)cc(C)n1[B-]2(F)F |c:4,7,t:1|
Structure
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