| Assay Method Information | |
| | In Vitro Enzyme Inhibition Assay |
| Description: | The cholinesterase assays were performed using colorimetric method reported by Ellman. The absorbance changes at 405 nm were recorded for 5 min with a microplate reader Digiscan 340T spectrophotometer. The reaction rates were compared and the percent inhibition due to the presence of test compounds was calculated. Inhibition curve was obtained for each compound. The compound concentration producing 50% of enzyme inhibition (IC50) was determined. |
| Affinity data for this assay | |
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