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Assay Method Information

Assay Name:  ligand-induced quenching of intrinsic tryptophan fluorescence
Description:  Fluorescence measurements were carried out with a Varian Cary Eclipse fluorescence spectrophotometer. Emission spectra were recorded from 330-360 nm upon excitation at 295 nm. All of the spectra were corrected for the background emission signal from both the buffer and the unbound ligand and repeated in triplicate. The equilibrium dissociation constant could be obtained from fitting the fractional fluorescence intensity data to a single site binding equation using nonlinear regression analysis.
Affinity data for this assay
 

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Last update November 1, 2007
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