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Assay Method Information

Assay Name:  Receptor Binding Assay
Description:   120 μL membrane (5 mg protein/well) was incubated with 15 μL of [125I]-CGP42112A and 15 μL of compound at RT for 1.5 hrs. The binding reaction was stopped by rapid filtration through Unifilter GF/C plates (presoaked in 0.3% (v:v) BSA). Plate was washed three times with ice cold wash buffer. The filtration plates were dried at 37 C. overnight. 50 μL of scintillation cocktail was added to each well. Radioactivity was determined using MicroBetaTriluxmicroplate scintillation counter.
Affinity data for this assay
 

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Last update November 1, 2007
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