Assay Method Information | |
| PKB In Vitro Kinase Assay |
Description: | An ELISA-based assay has been developed for the serine/threonine kinase, PKB-alpha. The assay utilizes an N-terminally biotinylated substrate peptide. When this substrate is phosphorylated by the kinase, the product is recognized by a substrate/sequence-specific antibody, which binds to particular phosphorylated serine residues. The amount of phosphorylated peptide was measured by the enzyme-catalyzed ABTS conversion and photometrical measurement at 405 nm. Calculation of IC50 values was performed with ActivityBase. |
Affinity data for this assay | |
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