| Assay Method Information | |
| | Fluorescence Polarization Competitive Binding Assay |
| Description: | A quantitative in vitro binding assay using the fluorescence polarization (FP) based method was developed and used to determine the binding affinity of Smac mimetics to XIAP protein. The polarization values were measured after 3 hrs of incubation when the binding reached equilibrium. IC50 values, the inhibitor concentration at which 50% of bound peptide is displaced, were determined from a plot using nonlinear least-squares analysis. Curve fitting was performed using Prism (GRAPHPAD). The binding affinity (Ki) of tested compound was calculated using the equation developed for computing the Ki value in FP-based binding assays. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |