Assay Method Information | |
| p38alpha Kinase Enzyme Assay |
Description: | IC50 is the inhibitor concentration, which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from [gamma-33P] labeled ATP to the substrate. Use of 33P-labeled ATP allows this transfer to be monitored by scintillation counting, and IC50s were calculated from replicate curves using GraphPad Prizm software. |
Affinity data for this assay | |
---|---|
If you find an error in this entry please send us an E-mail |