| Assay Method Information | |
| | Fluorescence Polarization Assay |
| Description: | Fluorescence polarization (FP) binding assay is utilizing the intrinsic property of the fluorescent probe, which will have low polarization value when free in solution. On binding to a target, the polarization of the probe will increase. Reaction mixtures of full length STAT3 alpha and the probe were placed in wells of a 96-well microtiter plate. The test peptide solution was added to each well. Fluorescence polarization was then read in a Tecan Polarian plate reader. The IC50 values were obtained from nonlinear regression analysis in the one-site competition mode. Peptides were assayed three times using three separate Stat3-probe preparations. IC50 values are reported as the mean of three IC50 values +/- the standard deviation. |
| Affinity data for this assay | |
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