| Assay Method Information | |
| | Enzyme Inhibition Assay |
| Description: | Fluorescence was monitored on a SPEX Fluorolog-2 spectrofluorometer with the excitation and emission wavelengths set at 380 and 440 nm, respectively. The Ki values were obtained from a graph of 1/Vs versus [I] by measuring the initial rate of substrate hydrolysis (Vs) in the presence of varying concentrations of inhibitor and at substrate concentrations kept well below Km. However, in most cases, nonlinearity in the initial portion of the progress curves indicated the presence of a slow inhibition process, and the data were analyzed as described in Biochemistry 1992, 31, 12571-12576. |
| Affinity data for this assay | |
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