| Assay Method Information | |
| | Enzyme Inhibition Assay |
| Description: | The inhibitory activity of the compounds against purified 15-LO enzyme was determined using a standard colorimetric assay in which the lipid hydroperoxide product (13-hydroperoxyoctadecadienoic acid) oxidizes Fe2+. The Fe3+ forms a chromophore with xylenol orange that absorbs strongly at 560 nm. Inhibitory activity was compared to an uninhibited (maximal) reaction to yield % inhibition (compound concentration in which enzyme activity is reduced by 50% is termed the IC50). |
| Affinity data for this assay | |
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