Assay Method Information

Assay Name:  FAAH Inhibition Assay
Description:  The effect of the test compounds on the enzymatic hydrolysis of [3H]anandamide was evaluated by using rat brain homogenate preparations. [3H]Ethanolamine produced from [3H]AEA hydrolysis was used to calculate FAAH activity and was measured by scintillation counting of the aqueous phase after extraction of the incubation mixture. Data are expressed as the concentration exerting a half maximal inhibition (IC50).
Affinity data for this assay
 

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