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Assay Method Information

Assay Name:  Peptidase Assay
Description:  Compound potency against the peptidase activity of LTA4 hydrolase was measured by inhibition of the hydrolysis of L-alanine-p-nitroanilide to L-alanine and highly colored nitroaniline. Formation of colored nitroaniline was measured by the increase in absorbance at 405 nm. Spontaneous hydrolysis of the substrate was corrected for by subtracting the absorbance of control incubations without enzyme. The IC50 values were determined from dose-response curves by non-linear fit of data to the 4-parameter fit equation. At least two independent determinations were made for each IC50 value.
Affinity data for this assay
 

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Last update November 1, 2007
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