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Assay Method Information

Assay Name:  Mtb EHB Inhibition Assay
Description:  Enzyme activity was measured by monitoring the appearance of fluorescent product, 6-methoxy-naphthaldehyde (ex@330 nm and em@ 465 nm) on a SpectraMax M2 microplate reader (Molecular Devices, Sunnyvale, CA). Assays were performed in triplicate. IC50 values were determined by regression of at least five datum points, with a minimum of two datum points in the linear region of the curve on either side of the IC50 values.
Affinity data for this assay
 

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Last update November 1, 2007
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