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Assay Method Information

Assay Name:  HTRF Cofactor Peptide Recruitment Assay
Description:  Polyhistidine-tagged human LXR ligand-binding domain was mixed with the test compound, biotin-SRC1 peptide, streptavidin-allophycocyanin, and europium labeled anti-polyhistidine antibody in reaction buffer, and the mixture was incubated in a 384-well assay plate format. Time-resolved fluorescence was measured at 615 and 665 nm on a LJL Analyst plate reader. The ratio of 665/615 was used to calculate EC50 values of test compounds.
Affinity data for this assay
 

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Last update November 1, 2007
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