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Assay Method Information

Assay Name:  ADAMTS-5 Enzyme Inhibition Assay
Description:  The ADAMTS-5 (Aggrecanase-2) assay was performed using fluorescent peptide substrate WAAG-3R and the recombinant Agg-2 pre-incubated with various concentrations of test compound. The reaction was initiated by addition of the substrate. Fluorescence was measured at excitation of 325 nm and emission of 405 nm with a fluorometric plate reader (Fluostar-P; BMG Lab Technologies).
Affinity data for this assay
 

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Last update November 1, 2007
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