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Assay Method Information

Assay Name:  Pim Kinase Dose-Response Assay
Description:  The activity of Pim kinase was measured in a homogeneous luciferase assay using GST-Pim, biotinylated peptide substrate and a luciferin-luciferase detection reagent to quantify residual ATP. Test compounds were serially diluted for the 10-point concentration response. The assay was performed in 96-well half-area, white, nonbinding surface microtiter plates (Corning, catalog no. 3642). Following incubation, ATP detection reagent was added to the assay plates and the relative light unit (RLU) signal was measured on the Analyst (Molecular Devices) in luminescence mode. The RLU signals were converted to percent of control in dose-response curves. The IC50 was determined as the inflection point parameter.
Affinity data for this assay
 

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Last update November 1, 2007
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