| Assay Method Information | |
| | DHODH Inhibition Assay |
| Description: | The assays were carried out by using a colorimetric DCIP method, which uses the colorimetric reagent 2, 6-dichlorophenolindophenol as the final electron acceptor. DCIP reduction is stoichiometrically equivalent to oxidation of dihydroorotate. Changes in absorbance were quantified on a plate reader and data were analyzed to measure the reduction of DCIP as a decrease in absorbance at 600 nm. For the determination of the IC50 values (concentration of inhibitor required for 50% inhibition) different inhibitor concentrations were applied. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |