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Assay Method Information

Assay Name:  Enzyme Inhibition Measurements
Description:  Inhibition assays were done in black 96-well plates by adding test compounds to the respective enzyme in assay buffer. Plates were incubated at room temperature for 1 h, and enzymatic activity was measured after addition of substrate in a Spectramax Gemini fluorescence plate reader (Molecular Devices, Sunnyvale, CA). IC50 values were calculated using the Microsoft Excel extension XL-fit.
Affinity data for this assay
 

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Last update November 1, 2007
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