Assay Method Information

Assay Name:  LXRbeta Binding Assay (IC50) and LAFbeta Functional Assay (EC50)
Description:  Binding reaction was initiated by adding a dilution series of test compound in tracer solution to LXRbeta coated flash plates. Each concentration of test compound was analyzed in duplicate. Bound radioactivity was measured in a Wallac Microbeta normalized for flashplates. LAFbeta assay determined the agonist potency of test compounds and their efficacy relative to T0901317 in CHO cells stably transfected with hLXRbeta using secreted alkaline phosphatase as the reporter gene, driven by multiple response elements for LXRbeta.
Affinity data for this assay

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