| Assay Method Information | |
| | Protease Inhibition Assay |
| Description: | Ki values were determined by using a fluorescent substrate (DABCYL-gamma-Abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS). All incubations were performed at 30°C in 0.1 M sodium acetate 1 M NaCl 1 mM DTT 1 mM EDTA 3% DMSO (pH 5.0) by using 5 uM substrate and the indicated enzyme concentrations ([E]). The enzyme concentration (A280) was determined by absorption measurements. Km values, including standard deviations, and calculated values of the active enzyme concentrations were obtained by nonlinear regression analysis. |
| Affinity data for this assay | |
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