Assay Method Information

Assay Name:  Inhibition Assay Protocol A
Description:  Threefold dilution series of inhibitors in assay buffer were prepared in wells of a microtiter plate (final concentration stating from 2uM), and TAMRA-Kemptide was added to each well. Separately, a mixture containing ATP (final concentration 5mM) and Aurora A or Aurora A:TPX2(1-43) (final concentrations 1.2nM and 400nM, respectively) was prepared. The microtiter plate and the mixture solution were preincubated for 15 min at 30 C, then the reaction was started by transfer of the mixture into each well of the microtiter plate. The reaction was stopped after 60 min incubation at 30 C by 20-fold dilution into phosphoric acid (75mM).
Affinity data for this assay
 

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