Assay Method Information

Assay Name:  IC50 Determination
Description:  To determine the IC50 values for CB29 and its analogues, propionaldehyde was used as the substrate for ALDH1A1 and ALDH2 and benzaldehyde was used as the substrate for ALDH3A1. The assays were performed at various concentrations of inhibitors, ranging from 50 nM to 250 uM, following 1 min preincubation. All reactions were initiated by the addition of the aldehyde substrate.
Affinity data for this assay
 

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