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Assay Method Information

Assay Name:  HIV-1 protease inhibition assay
Description:  The reaction mixture contained 2 µL of protease and 2 µL of inhibitor (or DMSO as a control) and was incubated for 20-30 min at room temperature prior to the substrate cleavage reaction. 150 µL of a 1 µM substrate were used in substrate buffer (0.1 M sodium acetate, 1 M sodium chloride, 1 mM EDTA, 1 MM DTT, 2% DMSO, and 1 mg/mL BSA with an adjusted pH of 4.7).
Affinity data for this assay
 

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Last update November 1, 2007
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