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Assay Method Information

Assay Name:  Aurora B Kinase Assay
Description:  The kinase assay was performed using the EMD Millipore KinaseProfiler service assay protocol. Aurora B kinase was supplied by EMD Millipore Corp. The substrate for phosphorylation, AlaLysArgArgArgLeuSerSerLeuArgAla, was 30 ÁM and the concentration of ATP was 10 ÁM. All experiments were repeated three times at four different concentrations (0, 5, 10, and 40 ÁM).
Affinity data for this assay
 

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Last update November 1, 2007
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