| Assay Method Information | |
| | Paraoxonase Activity Assay |
| Description: | Paraoxonase enzyme activity was determined at 25 °C with paraoxon (1 mM) in 50 mM glycine-NaOH (pH 10.5) containing 1 mM CaCl2. The enzyme assay was based on the estimation of p-nitrophenol at 412 nm. Assays were performed using a spectrophotometer (CHEBIOS UV-VIS, Fullerton, CA). The molar extinction coefficient of paranitrophenol (Ɛ = 18 290/M/cm at pH 10.5) is used for thecalculation of enzyme activity. One enzyme unit was defined as the amount of enzyme that catalyzes the hydrolysis of 1 µmol of paraoxon at 25 °C (J. Chromatogr. B. 2006, 836:15-21). |
| Affinity data for this assay | |
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