Assay Method Information

Assay Name:  α-Glucosidase inhibition assay
Description:  α-Glucosidase inhibition assay was performed spectrophotometrically. α-Glucosidase from Saccharomyces cerevisiae (Sigma-Aldrich) was dissolved in phosphate buffer (pH 6.8, 50 mM). Test compounds were dissolved in DMSO. In 96-well microtiter plates, 20 µL of test sample, 20 µL of enzyme (20 mU/mL) and 135 µL of buffer were added and incubated for 15 min at 37 °C. After incubation, 25 µL of p-nitrophenyl-α-D-glucopyranoside (2 mM, Sigma Aldrich) was added and change in absorbance was monitored for 20 min at 400 nm. Test compound was replaced by DMSO (10% final) as control. Acarbose (Sigma-Aldrich) was used as a standard inhibitor. The assays were done in triplicate.
Affinity data for this assay
 

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