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Assay Method Information

Assay Name:  ALK Kinase Biochemical Assay
Description:  BioKinase buffer was composed of 50 mM HEPES pH 7.5 containing 1 mM MnCl2, 5 mM MgCl2, 1 mM DTT, 3 microM Na3VO4, and 0.2 mg/mL BSA. 3x KB is buffer of the same composition and pH as KB, but with three times the concentration of each component. The kinase assay was run with a final enzyme concentration of 20 nM, in the presence of 8 microM ATP, 1 nM 33P-γ-ATP and 2 microM MBP. The MPB was purchased from Sigma-Aldrich, St. Louis, Mo., USA.
Affinity data for this assay
 

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Last update November 1, 2007
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