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Assay Method Information

Assay Name:  Homogeneous Time-resolved Fluorescence (HTRF) Assay
Description:  The assay was using baculovirus-expressed recombinant protein kinase and biotinylated poly-Glu-Ala-Tyr substrate. HTRF is based on the proximity of a europium cryptate (donor fluorophore)-labeled antiphosphotyrosine antibody and streptavidin labeled with XL665 (acceptor fluorophore) that have been brought together by a specific binding reaction. When the two entities come into close proximity and upon excitation, energy transfer occurs and XL665 re-emits at 665 nm
Affinity data for this assay
 

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Last update November 1, 2007
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