| Assay Method Information | |
| | LanthaScreen Kinase Binding Assay |
| Description: | This assay is based on the binding and displacement of Alexa-fluor 647-labeled ATP-competitive kinase inhibitor tracer 236. Binding of the tracer is detected using a europium-labeled anti-tag antibody that binds to the purified recombinant tagged Itk protein. Binding of the tracer 236 is reversible and rapid, and simultaneous binding of the tracer and antibody results in fluorescence resonance energy transfer (FRET). Displacement of the tracer upon inhibitor binding results in a decrease of FRET. The assay can be read continuously, enabling rates of binding (and displacement) to be monitored. |
| Affinity data for this assay | |
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