| Assay Method Information | |
| | α-Glucosidase Inhibition Assay |
| Description: | Briefly, the sample solution (25 μL, 1.0 mM solution in DMSO) was mixed with enzyme solution (25 μL, 0.5 U/mL prepared in 0.1 M phosphate buffer, pH 6.8). The mixture was incubated at 37 ± 1 °C for 10 min to allow inhibition of enzyme by test samples. After the incubation, the substrate p-nitrophenyl α-D-glucopyranoside (25 μL, 0.5 mM, 0.1 Mphosphate buffer, pH 6.8) was added to the mixture and allowed to incubate at 37 ± 1 °C for 30 min. The reaction was then terminated by the addition of aqueous solution of sodium carbonate (100 μL, 0.2 M). Finally, absorbance was measured with a microplate reader at 405 nm. The uninhibited enzyme solution was taken as blank and an appropriate DMSO control was used. Acarbose was used as the positive control. The concentration of DMSO in the final solution was maintained below 5.0% so that the enzyme activity was not destroyed. |
| Affinity data for this assay | |
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