| Assay Method Information | |
| | In Vitro Src Kinase Inhibition Test |
| Description: | This assay determines the ability of test compounds to inhibit Src kinase activity that catalyzes the transfer of the terminal phosphate to the immobilized poly (Glu,Tyr) substrate detected using anti-phosphotyrosine monoclonal antibody conjugated to alkaline phosphatase. IC50 values for compound enzyme inhibition were obtained using KC3 Kineticalc software (Bio-Tek Instruments) following subtraction of the blank values. |
| Affinity data for this assay | |
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