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Assay Method Information

Assay Name:  Enzymatic Assay
Description:  Lysosomal NAAA protein preparation were obtained by homogenizing male Sprague-Dawley rat lungs (Charles River) in 20 mM Tris-HCl buffer pH 7.4 containing 0.32M sucrose. Samples were centrifuged at 800g for 15 minutes at 4 C. Supernatants were then centrifuged at 12,000 g for 30 minutes at 4 C. Pellets were then resuspended in PBS pH 7.4 and subjected to a freeze/thaw cycle at −80 C. The suspension was finally centrifuged at 105,000g for 1 hour at 4 C. The supernatant was then used in the enzymatic assay.
Affinity data for this assay
 

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Last update November 1, 2007
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