| Assay Method Information | |
| | Urokinase Amidolytic Assay |
| Description: | Buffer and 0.1 mL of enzyme solution was added to 0.2 mL of examined compounddissolved in 0.15 M NaCl (as control 0.15 M NaCl). The buffer and the enzyme solution included: Tris buffer − 0.6 mL (pH 8.8), enzyme: urokinase (50 units/mL), synthetic substrate: S-2444 (0.1 mL, 3 mM). The mixture was incubated for 3 min at 37°C. After 20 min of incubation, the reaction was stopped by adding 0.1 ml of 50% acetic acid, and the absorbance of the released p-nitroaniline was measured at 405 nm (Spekol 1300, AnalyticJena). |
| Affinity data for this assay | |
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