| Assay Method Information | |
| | AChE and BuChE Activity Assay |
| Description: | Seven different concentrations of the synthesized compounds in the range 10^−3-10^−9 M were measured at 412 nm. All the assays were under 0.1 M KH2PO4/K2HPO4 buffer (pH = 8) using a Varian Cary 50 Spectrophotometer. Enzyme solutions were prepared to give 2 units/mL in 2 mL aliquots. The assay medium contained phosphate buffer, pH 8.0 (1 mL), 50 μL of 0.01 M DTNB, 10 μL of enzyme, 50 μL of acetylthiocholine iodide (ATCh) and 50 μL of the test compound solution. ATCh was added to the assay medium after 10 min of incubation time. The activity was determined by measuring the increase in absorbance at 412 nm for 1 min interval at 37 ± 0.2°C. For determining the blank value, additionally 50 μL buffer replaced the enzyme solution. In vitro the BuChE assay uses the similarmethod to that described above. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |