Assay Method Information | |
| CDKs Assay |
Description: | In vitro kinase assays using synthetic peptides and purified enzymes were incubated at 30°C for 45 min in buffer that contained 50 uM ATP, and diluted compounds. The reactions were terminated and the phosphorylated peptides were trapped in a filter. 33P incorporation was measured with a Top Count (Packard). IC50 is the inhibitor concentration which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate from 33P labeled ATP to peptide substrate. |
Affinity data for this assay | |
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