Assay Method Information | |
| Receptor Binding Assay |
Description: | The affinity of the test compounds for the GABA gated Cl-channel (GABA.A receptor complex) in the rat cerebral cortex was determined in a radioligand binding assay (Lewin et al,. 1989). Membrane homogenates of cerebral cortex are incubated for 120 min at 22° C. with 3 nM [35S]TBPS (-t-butylbicyclophosphoorothionate) in the presence and absence of the test compound.Following incubation, the samples are filtered rapidly under vacuum through glass fiber filters and rinsed several times with ice-cold buffer using a 96-sample cell harvester. The filters are dried then counted for radioactivity in a scintilliation counter using a scintillation cocktail (Microscint 0, Packard). Each compound was tested at several concentrations to calculate the IC50 values of the affinity to the GABA.A receptor complex in cerebral cortex of rats. |
Affinity data for this assay | |
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