Assay Method Information

Assay Name:  In Vitro Pepsin Inhibition Assay
Description:  In vitro potencies of compounds against purified porcine pepsin were determined by its cleavage of substrate hemoglobin. After incubation, proteins were precipitated with perchloric acid and the absorbance of the supernatant was measured at a wavelength of 280 nm.
Affinity data for this assay
 

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