| Assay Method Information | |
| | HTRF Assay of Receptor Tyrosine Kinase and Cellular KDR Phosphorylation Assay |
| Description: | The assay uses purified enzyme interacting with biotinylated peptide substrate. HTRF is based on the proximity of europium cryptate (donor fluorophore)-labeled antiphosphotyrosine and streptavidin labeled with APC(allophycocyanin), which have been brought together by a binding reaction. When the two entities come into close proximity and upon excitation, energy transfer occurs and APC re-emits at 665 nm. IC50 are the means of two determinations with seven concentrations of the test compounds. Cellular EC50/IC50 values were obtained from ELISA assay, measuring KDR phosphorylation in NIH3T3 cells stably transfected with full length human KDR. |
| Affinity data for this assay | |
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