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Assay Method Information

Assay Name:  Inhibition Assay Protocol B
Description:  Threefold dilution series of inhibitors in assay buffer were prepared in wells of a microtiter plate (final concentration stating from 2uM). Subsequently, a mixture of TAMRA-Kemptide and Aurora A or Aurora A:TPX2(1-43) (final concentrations 1.2 and 400nM, respectively) was added to each well. Separately, a solution containing ATP (final concentration 5mM) was prepared. The microtiter plate and the ATP solution were preincubated for 15 min at 30 C, and the reaction was started by transfer of ATP into each well of microtiter plate. The reaction was stopped after 60 min incubation at 30 C by 20-fold dilution into phosphoric acid (75mM).
Affinity data for this assay
 

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Last update November 1, 2007
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