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Assay Method Information

Assay Name:  Isothermal Titration Calorimetry Assays
Description:  Titrations were performed using a VP-ITC or ITC200 calorimeter from Microcal (Northampton, Mass.). BCL-XL was used at concentrations between 25 and 100 μM in 20 mM sodium phosphate buffer (pH 7.4) and 5-10% DMSO. Titrants were used at concentrations 10-15 of the protein in the same buffer. Titrations were carried out at 25 C. Data were analyzed using Microcal Origin software provided by the ITC manufacturer (Microcal, Northampton, Mass.).
Affinity data for this assay
 

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Last update November 1, 2007
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