| Assay Method Information | |
| | α-Chymotrypsin Inhibition Kinetic Assay |
| Description: | The change in optical density per minute (OD/min) was obtained by incorporating various concentrations of compounds over a range of substrate (SPpNA) concentrations between 0.4 mM and 3.2 mM. Reciprocal of the rate of the reactions against the reciprocal of the substrate concentration as Lineweaver-Burk plot (and its secondary plot; slope versus compound concentration); the Dixon plot (and its secondary plot; slope versus reciprocal of compound concentration) and then Hanes-Woolf plot were plotted. |
| Affinity data for this assay | |
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